Dominic Solanke, James Maddison, Destiny Udogie and Mateus Fernandes are all fit enough to be in the squad for Spurs. However, Micky van de Ven, Pedro Porro and Pape Matar Sarr will miss the game because of injury.
De Zerbi has named Van de Ven as his new captain for the 2026-27 campaign, and also listed Porro, Ben Davies, Maddison and Archie Gray as replacement skippers.
The Italian said it was "not right to speak about players until they are official" when asked about Tottenham's links to Manchester City's Savinho.
De Zerbi confirmed he and the board are "working very well" in the closing stages of the transfer window, during which six players have joined so far.
He explained: "We have spoken a lot of times in the summer. It is a connection. It is the connection between the people inside the club and I think we are closing out the first target we ever had in our plan."
De Zerbi said Spurs "can't forget what happened" last season and that it was a "big lesson" for his side. "We're building a new project. The first target is very clear: to find the soul of the team. The second target is to improve in our organisation with and without the ball. Our style has to be the key to our season."