Cristian Romero has an abductor strain and will miss the match, alongside fellow defender Destiny Udogie, who didn't travel because of a "knee irritation".
However, Frank said the club are "assessing" the pair day by day and fans should not be concerned about the injury to their captain.
On how Xavi Simons is settling after his summer move: "He's a very good player coming into a new country, new club, new city. We're all getting judged every game but we need to see the bigger perspective. There were some good bits - asking him to arrive in the box, and he did. It's constantly going in the right direction."
Brennan Johnson is yet to score this Champions League campaign and Frank believes getting the best out of the forward is about "making it fit".
The boss said: "He's a very important player. Such important goals last season and top scorer. Started the season well here so it's down to making it fit. Brennan can play both left and right - Mo [Kudus] has done good on the right and, on the left, Wilson [Odobert] has done well."
Spurs drew 2-2 with Bodo/Glimt in their last European away game but Frank is confident in his travelling team: "Away form has been very good. We've been good in the football foundation. We have a good base and middle and low block. We're one of the most progressive teams in the final third. We need to do the same tomorrow against a very good team."