The team became the first and, so far, only non-league side to win the competition- and the winners had a real Ayrshire flavour to them.
Spurs faced Sheffield United in the final and a 2-2 draw meant a replay, where Tottenham eventually ran out 3-1 winners on April 27, 1901.
Player/manager John Cameron hailed from Ayr, as did David Copeland. Both men started both finals, with Cameron scoring in the replay.
The Ayrshire ties not stop there, as winners Sandy Brown and Sandy Tait were both born and raised in the former mining village of Glenbuck- most famous for being the birthplace of Bill Shankly.
Brown's impact is still remembered at Spurs to this day, as he became the first man to score in every round of the competition, including both goals in the first final, and one more in the replay.
The Glenbuck boys' impact was recently detailed in an informative article by Tottenham Hotspur.
In it, it explains how local stories state that the boys brought the trophy back to Glenbuck at the time, and displayed it on the Co-operative store window for the community to admire.
A dedicated sign at the Glenbuck Heritage Village pays tribute to Tait and Brown's influence on the FA Cup-winning side, as well as football in that part of the country as a whole.
That FA Cup triumph would prove to be the only major honour any of the men won, and set Spurs into a new era, entering the football league a few years later and winning seven more FA Cups since.