Wearing a black coat and matching sunglasses, the singer was in the stands as the home team lost 2-0 on Sunday afternoon.
It came a day after the 67-year-old watched her twin daughters, Estere and Stella, play in an under-14s Spurs academy game.
Tottenham’s senior team slipped to defeat against Chelsea thanks to goals from Keira Walsh and Alyssa Thompson either side of half-time. The result ended the third-placed visitors’ run of back-to-back losses, while Spurs stayed fifth in the table.
The Queen of Pop has six children: the twins, who were adopted from Malawi in 2017, her son David Banda, who she adopted in 2006, daughter Mercy James, who she also adopted from Malawi in 2009, Rocco Ritchie, who she shares with ex-husband, filmmaker Guy Ritchie, and her eldest child, daughter Lourdes, from a relationship with actor Carlos Leon.
Madonna, winner of seven Grammy Awards, saw her career peak in the 1980s with hits such as Holiday, Vogue and Material Girl. She had 13 number one singles and 12 number one albums in the UK, including Like A Virgin, Confessions On A Dance Floor and Like A Prayer.